Faculty Biography
Address:
Physicians & Surgeons
Room 7-517
630 West 168th St.
New York, NY 10032
Phone: (212) 305-1673
djg5@columbia.edu
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Education and Training
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- Pharmacology
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-Program Director - Graduate Program in Pharmacology
-Course Director - Neural Science (1st Year Medical & Dental Students)
-Co-course Director - Molecular Mechanisms in Synaptic Transmission and the Control of Transmitter Release (advanced graduate course of the doctoral program in neurobiology and behavior)
-Lecturer - Pharmacology Course (2nd Year Medical & Dental Students)
-Faculty Member - Predoctoral training programs in pharmacological sciences and in Neurobiology and Behavior
-Faculty Member - Postdoctoral training program in Neurobiology and Behavior
Synaptic plasticity in Aplysia – collaboration with Dr. Samuel Schacher of Center for Neurobiology and Behavior
Functioning of dynein and related proteins in the neuronal growth cone – collaboration with Dr. Richard Vallee of Department
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Daniel J. Goldberg, Ph.D.
Professor of Pharmacology and Neuroscience
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Research
Summary
Growth of neuronal processes and formation of synapses during development and learning
We are interested in cellular and molecular mechanisms underlying the growth of neuronal processes during development and after injury. We have focused on the growth cone, the specialized ending of a growing process whose interactions with environmental cues are critical in regulating the rate and direction of growth. We are seeking to define the intracellular changes within the growth cone caused by certain important cues and the signal transduction mechanisms that elicit those changes. We focus on the actin filament and microtubule systems of the growth cone. We also want to apply this knowledge to the analysis of the synapse formation that occurs during learning in the adult animal. We are examining the morphological events underlying the formation of new synapses and the growth of neuronal arbor associated with certain types of long-term synaptic plasticity, using low light level fluorescence imaging of live cells. We are currently examining the contribution to this growth of changes in protein synthesis and in the delivery of mRNA species to the synapse.
For our experiments, we use both vertebrate and invertebrate neurons studied in culture. Many of our studies involve the use of high resolution video microscopy. In addition, we use biochemical techniques such as gel electrophoresis and blotting detections (Western, Northern), single cell microinjection, and molecular techniques such as antisense depletion of particular proteins.
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Selected Publications:
1. Grabham, P.W., Reznik, B. and Goldberg, D.J. (2003) Microtubule- and Rac 1-dependent F-actin in growth cones J. Cell Sci
116:3739-48
2. Grabham, P.W., Foley, M., Umeojiako, A. and Goldberg, D.J. (2000) Nerve growth factor stimulates coupling of b1 integrin to distinct transport mechanisms in the filopodia of growth cones. J. Cell Sci
113:3003-3012
3. Hatada, Y., Wu, F., Sun, Z-Y., Schacher, S. and Goldberg, D.J. (2000) Presynaptic morphological changes associated with long-term synaptic facilitation are triggered by actin polymerization at pre-existing varicosities J. Neurosci
20:RC82:1-5
4. Tang, D. and Goldberg, D.J. (2000) Bundling of microtubules in the growth cone induced by laminin Molec. Cell. Neurosci
15:303-313
5. Grabham, P.W. and Goldberg, D.J. (1997) Nerve growth factor stimulates the accumulation of b 1 integrin at the tips of filopodia in the growth cones of sympathetic neurons J. Neurosci
17:5455-5465
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Honors and Awards
Alfred P. Sloan Research Fellowship
Irma T. Hirschl Career Scientist Award
Charles W. Bohmfalk Award for Distinguished Contributions to Teaching
Committees
Curriculum Committee of the College of Physicians & Surgeons
First Year Class Committee of the College of Physicians & Surgeons
First Year Class Committee of the School of Dental and Oral Surgery
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